Antibody-antigen complexes.

Antigen-Antibody Immune Complexes

Studies on Antigen-antibody Complexes

Solid phase immunoadsorbents were prepared by coupling antigens to agarose. With this technique specific antibodies were easily isolated in large amounts. The gammaG-globulin class of antibodies isolated in this manner were not denatured as judged by their normal biological half-life in rabbits. Soluble immune complexes at fivefold antigen excess were prepared from isolated specific antibodies ...

Soluble complexes of antigen and antibody fragments.

The insolubility of antigen-antibody aggregates formed at most proportions of reactants has prevented physicochemical study of these systems of interacting proteins under conditions in which they remain associated. Only when the antigen is present in great excess and soluble complexes are formed have the products been examined by ultracentrifugation (14)) moving boundary electrophoresis (l-3), ...

The Structure of Antigen-antibody Complexes

Negatively stained aggregates of antigen (polyoma or verruca vulgaris virus) and antibody (from rabbit or goat) were examined in the electron microscope. The antibody molecules appeared as cylindrical rods (often, but not always, showing a beaded appearance) with a long axis of 250 to 270 A and a short axis of 35 to 40 A. The combining sites were at the opposite short ends of the antibody molec...

The Biological Activity of Soluble Antigen-antibody Complexes

Soluble BSA-anti-BSA complexes, formed in antigen excess, give immediate skin reactions in normal guinea pigs. The mechanism of the reaction is not that of passive or reversed passive anaphylaxis. The complex itself is toxic. Skin activity of the complex depends on its composition. It has become obvious that the complex composed of two antigen molecules and one antibody molecule, (Ag(2)Ab), doe...